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Turn your biochemistry notes into glycolysis MCAT practice questions

Upload your PDF, slides or notes and DeckOwl writes multiple-choice, true/false and short-answer questions on glycolysis, each with an explanation, for MCAT review.

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How glycolysis questions help with MCAT biochemistry

Glycolysis shows up in MCAT biochemistry passages because it connects to enzyme regulation, metabolic disorders and oxygen availability. Working through glycolysis MCAT practice questions asks you to apply the pathway rather than recite it, which is the skill the exam rewards.

Net yield of 2 ATP and 2 NADH per glucose
Regulation at phosphofructokinase-1 by ATP, AMP and fructose-2,6-bisphosphate
Fate of pyruvate with and without oxygen
Links to gluconeogenesis and the Cori cycle

Sample glycolysis MCAT practice questions with answers

Which glycolytic enzyme is the main control point and is activated by AMP and fructose-2,6-bisphosphate?Phosphofructokinase-1 (PFK-1). It catalyzes the committed step of glycolysis, so it is the key point of regulation.

What is the net ATP yield from glycolysis per glucose molecule?2 ATP. Two ATP are invested in the early steps and four are produced by substrate-level phosphorylation.

Under anaerobic conditions, which enzyme regenerates NAD+ so glycolysis can continue?Lactate dehydrogenase. It reduces pyruvate to lactate, oxidizing NADH back to NAD+ for glyceraldehyde-3-phosphate dehydrogenase.

True or false: glucose-6-phosphate allosterically inhibits hexokinase.True. Glucose-6-phosphate is the product of the reaction, and its buildup slows the enzyme, while liver glucokinase is not inhibited this way.

Which glycolytic step is catalyzed by pyruvate kinase, and what does it produce?Phosphoenolpyruvate to pyruvate, producing ATP by substrate-level phosphorylation. It is the final and irreversible step of glycolysis.

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Questions from your own material

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An explanation for every answer

Each question comes with an explanation that shows why the correct choice works and where the distractors go wrong.

Three question formats

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How it works

Upload your material

Add a PDF, DOCX, PPTX, plain text file or a photo of your glycolysis notes.

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Practice and review

Answer each question, read the explanation, and repeat weak spots until the pathway holds up under pressure.

Practice questions work best when they come from your own course material. DeckOwl reads your slides or notes and writes questions around the enzymes, intermediates and ATP accounting your instructor actually covered.

Focus on the three irreversible steps catalyzed by hexokinase, phosphofructokinase-1 and pyruvate kinase, along with what happens to pyruvate under aerobic and anaerobic conditions. Those are the points where questions tend to hinge.

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Frequently asked questions

What do glycolysis questions on the MCAT usually test?

They often test the energy accounting, the three irreversible enzymes, and how phosphofructokinase-1 is regulated. Expect questions that link glycolysis to pyruvate fate and gluconeogenesis.

How should I study glycolysis for the MCAT?

Learn the intermediates in order, then focus on the three irreversible steps and their regulators. Practice explaining why NAD+ regeneration matters when oxygen is limited.

Can I export these questions?

Yes, but exports are paid features. Plus includes PDF, DOCX, Anki and CSV export, and Pro adds QTI 2.1 and Moodle XML.

Do I need a credit card to start?

No, you can start without a credit card. The free plan includes 3 generations per month and studying in the browser.

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