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Turn your biochemistry notes into MCAT practice questions

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How to use biochem practice questions for the MCAT

Biochemistry carries a large share of the biological and biochemical foundations material on the MCAT, so MCAT biochem practice questions work best when they track the exact pathways and enzymes in your course. Generic question banks rarely match your lecture order, which is why many students end up re-reading slides instead of testing themselves.

Practice one pathway at a time before mixing topics across the whole course.
Read the explanation for every question, not only the ones you missed.
Track cofactors and regulatory enzymes separately, since they are common distractors.
Retest missed questions after a short gap to see what actually stuck.

Example MCAT biochem practice questions

Which enzyme catalyzes the committed, rate-limiting step of glycolysis?Phosphofructokinase-1 (PFK-1), which converts fructose-6-phosphate to fructose-1,6-bisphosphate and is the main regulated control point of the pathway.

In competitive inhibition, how do Km and Vmax change?Km increases and Vmax stays the same, because the inhibitor competes with substrate for the active site and high substrate concentration can overcome it.

What is the final electron acceptor in the electron transport chain?Oxygen, which accepts electrons at complex IV and combines with protons to form water, so the chain stops without it.

How many net ATP does glycolysis produce per glucose molecule?2 net ATP, since the pathway produces 4 ATP and uses 2 in the preparatory phase.

Which vitamin-derived cofactor is required by pyruvate dehydrogenase?Thiamine pyrophosphate, derived from vitamin B1, which is needed to decarboxylate pyruvate before it enters the citric acid cycle as acetyl-CoA.

Generate the full set

Built from your material

Questions come from the pathways and terms in your own course files, so you practice what your class actually covers.

Explanations for every answer

Each question shows why the correct answer is right and why the distractors are wrong, so missed questions become lessons.

Three question formats

Mix multiple-choice, true/false and short-answer questions to test recall, application and the reasoning behind enzyme and pathway problems.

How it works

Upload your notes

Add a PDF, DOCX, PPTX, plain text file or a photo of a textbook page covering the topic.

Choose a format and count

Pick multiple-choice, true/false or short-answer questions and how many you want to practice.

Answer and review

Work through the set, read the explanations, and repeat missed questions until the pathways stick.

Start by building a set from one unit, such as glycolysis or the citric acid cycle, and answer it without looking at your notes. Read every explanation, including for the questions you got right, and note which regulatory steps and cofactors you confused. Then retake the set a few days later to check what you retained.

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Frequently asked questions

What topics do biochem practice questions usually cover for the MCAT?

Common topics include enzyme kinetics, glycolysis, the citric acid cycle, oxidative phosphorylation, amino acid metabolism and nucleic acid processes. Your own notes determine which of these you generate questions on.

How should I study enzyme kinetics questions?

Learn how competitive, noncompetitive and uncompetitive inhibitors change Km and Vmax, then practice reading Lineweaver-Burk plots. Working several problems on the same concept is more useful than one long set.

Can I export my practice questions?

Exporting is a paid feature. PDF and DOCX export are included in Plus, and QTI 2.1 and Moodle XML export are included in Pro.

Is there a free plan?

Yes. The free plan includes 3 generations per month and lets you study in the browser, though it does not include exports. No credit card is needed to start.

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